Chromatographic Differences Between Radioiodinated Albumin Preparations and Normal Human Serum Albumin

Abstract

Radioiodinated human serum albumin preparations, representative of the preparations utilized in investigations of albumin metabolism in man, were chromatographed by a standard technique on anion-exchange (diethylamino-ethyl-) cellulose columns. The chromatographic distribution of the radioiodinated preparations were found to differ markedly from the distribution of native serum albumin. The possible causes of albumin modification such as isolation procedures, heat sterilization, iodination and radiation exposure are briefly reviewed.