Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA
- 1 January 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (3), 1687-1696
- https://doi.org/10.1093/nar/12.3.1687
Abstract
We report the complete nucleotide sequence of a human beta actin cDNA. Both the 5' and 3' untranslated regions of the sequence are similar (greater than 80%) to the analogous regions of the rat beta-actin gene reported by Nudel et al (1983). When a segment of the 3' untranslated region is used as a radiolabelled probe, strong hybridization to chick beta actin mRNA is seen. This conservation of sequences suggests that strong selective pressures operate on non-translated segments of beta actin mRNA.Keywords
This publication has 13 references indexed in Scilit:
- Strong homology in promoter and 3′-untranslated regions of chick and rat α-actin genesNature, 1983
- A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.Molecular and Cellular Biology, 1983
- The nucleotide sequence of the rat cytoplasmic β–actin geneNucleic Acids Research, 1983
- Nucleotide sequence divergence and functional constraint in mRNA evolution.Proceedings of the National Academy of Sciences, 1980
- Coexpression of a mutant β-actin and the two normal β- and γ-cytoplasmic actins in a stably transformed human cell lineCell, 1980
- The evolution of genes: the chicken preproinsulin geneCell, 1980
- Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probesCell, 1980
- Histone gene expression during sea urchin embryogenesis: Isolation and characterization of early and late messenger RNAs ofStrongylocentrotus purpuratus by gene-specific hybridization and template activityDevelopmental Biology, 1979
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Molecular Evolution of Myoglobin and the Fossil Record: a Phylogenetic SynthesisNature, 1973