The stimulus-induced release of 5-hydroxytryptamine and tryptophan from superfused rat brain synaptosomes
- 1 June 1982
- journal article
- research article
- Published by Springer Nature in Journal of Neural Transmission
- Vol. 53 (2-3), 223-230
- https://doi.org/10.1007/bf01243414
Abstract
The synthesis of 5-hydroxytryptamine (5-HT) from3H-tryptophan in rat forebrain synaptosomes was studied. Synaptosomes which had been preincubated with3H-tryptophan were perfused with Krebs solution and the release of tryptophan, 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) was determined. K+ depolarization induced a Ca2+-dependent release of 5-HT and tryptophan but had no effect on 5-HIAA efflux. This finding suggests that the release of tryptophan is unlikely to be a non-specific effect of depolarization.This publication has 14 references indexed in Scilit:
- The stimulus-induced release of unmetabolized 5-hydroxytryptamine from superfused rat brain synaptosomesJournal of Neuroscience Methods, 1981
- Differential effects of short and long term lithium on tryptophan uptake and serotonergic function in cat brainLife Sciences, 1980
- Effects of GABA, dopamine, and substance P on the release of newly synthesized 3H-5-hydroxytryptamine from rat substantia nigra in vitroNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1978
- Effets de la 5–6 dihydroxytryptamine sur les mecanismes de transport de divers neurotransmetteurs ou de leurs precurseurs au niveau des synaptosomes du mesencephale de rat.Biochemical Pharmacology, 1975
- A method for concurrent measurement of picomole quantities of acetylcholine, choline, dopamine, norepinephrine, serotonin, 5-hydroxytryptophan, 5-hydroxyindoleacetic acid, tryptophan, tyrosine, glycine, aspartate, glutamate, alanine, and gamma-aminobutyric acid in single tissue samples from different areas of rat central nervous systemAnalytical Biochemistry, 1975
- Newly synthesized norepinephrine accumulation in the readily releasable pool of a purified adrenergic vesicle fractionLife Sciences, 1975
- Preferential release of newly synthesized 3H‐acetylcholine from rat cerebral cortex slices in vitroBritish Journal of Pharmacology, 1973
- Serotonin synthesis with rat brain synaptosomes: Effects of serotonin and monoamineoxidase inhibitorsBiochemical Pharmacology, 1972
- ISOLATION OF NERVE ENDINGS FROM BRAIN - AN ELECTRON-MICROSCOPIC STUDY OF CELL FRAGMENTS DERIVED BY HOMOGENIZATION AND CENTRIFUGATION1962
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951