Phospholipids bound to acidic nuclear proteins in human B and T lymphocytes
- 1 August 1976
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 12 (2), 67-71
- https://doi.org/10.1007/bf01731551
Abstract
Summary Chromatin fractions (DNA, histones and nonhistone chromosomal proteins NHCP) have been isolated from human peripheral B and T lymphocytes using different methods and analyzed in order to identify their lipid content. While DNA and histone fractions do not reveal the presence of lipids, a 2% of phospholipids is present in the NHCP fraction. The phospholipids associated with NHCP present a constant relative ratio among sphingomyelin, phosphatidyl-choline and phosphatidyl-ethanolamine both in B and T lymphocytes, whichever are the extraction procedures employed. These findings are related to the possible derepressive role of phospholipids on DNA-dependent RNA synthesis.Keywords
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