Sequence requirements for nuclear location of simian virus 40 large-T antigen
- 6 September 1984
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 311 (5981), 33-38
- https://doi.org/10.1038/311033a0
Abstract
A point mutation in the simian virus 40 large-T gene, which was generated by mixed oligonucleotide mutagenesis and resulted in the conversion of Lys 128 to Thr, produced a large-T antigen that was detected in the cytoplasm but not the nucleus of cells. Deletions within the surrounding sequence Lys-128Lys-Lys-Arg-Lys-Val-Glu also produce cytoplasmic large-T and define a region of the protein involved in nuclear location.Keywords
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