Identification of coated vesicles in Saccharomyces cerevisiae.
Open Access
- 1 January 1984
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 98 (1), 341-346
- https://doi.org/10.1083/jcb.98.1.341
Abstract
Clathrin-coated vesicles were found in yeast, Saccharomyces cerevisiae, and enriched from spheroplasts by a rapid procedure utilizing gel filtration on Sephacryl S-1000. The coated vesicles (62-nm diam) were visualized by negative stain electron microscopy and clathrin triskelions were observed by rotary shadowing. The contour length of a triskelion leg was 490 nm. Coated vesicle fractions contain a prominent band with molecular weight of approximately 185,000 when analyzed by SDS PAGE. The presence of coated vesicles in yeast cells suggests that this organism will be useful for studying the function of clathrin-coated vesicles.Keywords
This publication has 17 references indexed in Scilit:
- Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles.Proceedings of the National Academy of Sciences, 1976
- Involvement of vesicle coat material in casein secretion and surface regeneration.The Journal of cell biology, 1976
- Protein transport: A selective membrane mechanismJournal of Supramolecular Structure, 1976
- Coated vesicles from pig brain: Purification and biochemical characterizationJournal of Molecular Biology, 1975
- Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dyeAnalytical Biochemistry, 1975
- Morphological evidence for de novo formation of plasma membrane from coated vesicles in exponentially growing cultured plant cells*1Experimental Cell Research, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coliBiochemistry, 1968
- FUNCTIONS OF COATED VESICLES DURING PROTEIN ABSORPTION IN THE RAT VAS DEFERENSThe Journal of cell biology, 1967
- LYSOSOMES AND GERL IN NORMAL AND CHROMATOLYTIC NEURONS OF THE RAT GANGLION NODOSUMThe Journal of cell biology, 1967