Mapping of the T and B cell epitopes of the Mycobacterium bovis protein, MPB70
- 22 December 1990
- journal article
- Published by Wiley in Immunology & Cell Biology
- Vol. 68 (6), 359-365
- https://doi.org/10.1038/icb.1990.49
Abstract
A clone coding for the entire gene for the Mycobacterium bovis protein antigen MPB70 was used to produce a series of overlapping subclones by making a series of deletions from the 3' end of the gene. The subclones expressed incomplete MPB70 proteins as fusions with glutathione-S-transferase. The insert DNA was sequenced to determine the extent of the deletion and the proteins expressed by the clones were examined for the presence of T cell and B cell epitopes. T cell epitopes were mapped by measuring the ability of recombinant antigens to stimulate gamma interferon (-IFN) production in a whole blood culture system. -IFN production was measured using a sandwich enzyme immunoasssay specific for bovine -IFN. B cell epitopes were mapped with a series of anti-MPB70 monoclonal antibodies using an indirect enzyme immunoassay.Keywords
This publication has 25 references indexed in Scilit:
- A sandwich enzyme immunoassay for bovine interferon‐γ and its use for the detection of tuberculosis in cattleAustralian Veterinary Journal, 1990
- Epitope mapping of the Mycobacterium bovis secretory protein MPB70 using overlapping peptide analysisJournal of General Microbiology, 1990
- Complete nucleotide sequence of immunogenic protein MPB70 fromMycobacterium bovisBCGFEMS Microbiology Letters, 1989
- Purification of a Major Mycobacterium bovis Antigen for the Diagnosis of Bovine TuberculosisScandinavian Journal of Immunology, 1989
- Identification of Mycobacterium bovis isolates using a monoclonal antibodyVeterinary Microbiology, 1988
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988
- Genes for the major protein antigens of the leprosy parasite Mycobacterium lepraeNature, 1985
- 3,3′,5,5′-Tetramethylbenzidine as an Ames Test Negative Chromogen for Horse-Radish Peroxidase in Enzyme-ImmunoassayJournal of Immunoassay, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970