The excretion of derivatives of certain carcinogenic and non-carcinogenic hydrocarbons in fowl bile

Abstract

The elimination, after intraven. inj. in the fowl, of 4 carcinogenic and 6 non-carcinogenic hydrocarbons was compared with that of 3:4-benzpyrene by the method of sampling bile from animals with enclosed cholecystostomy fistulae. The U.V. fluorescence of the fistula bile was examined, and any fluorescing material observed was extracted and analysed by fluorescence methods to establish the presence of any unchanged hydrocarbon or its derivative. Benzpyrene was eliminated in part unchanged, after intraven. inj. of 1-10 ml. of a 0.03% colloid; none of the other ten hydrocarbons was eliminated unchanged. 1:2:5:6-dibenzanthracene, cholanthrene, methylcholanthrene, anthracene, pyrene, 2[image]:6-and 2[image]:7-dimethyl-1:2-benzanthracene, and fluoranthene, like 3:4-benzpyrene, were eliminated as fluorescent derivatives, and 3:4-benzphenanthrene and phenanthrene gave rise to no fluorescent derivative in the bile.