The Nucleotide Sequence of 5S Ribosomal RNA from Slime Mold Physarum polycephalum

Abstract

The nucleotide sequence of 5S ribosomal RNA from plasmodia of the slime mold Physarum polycephalum was determined as pppGGAUGCGGC$C10$AUACUAAGG$A20$GAAAGCACC$U30$CAUCCCGUC$C40$GAUCUGAGA$A50$GUUAAGCUC$C60$UUCAGGCGU$G70$GUUAGUACU$G80$GGGUGGGGG$A90$CCACCUGGG$A100$AUCCCACGU$G110$CUGCAUUCU$U120$ by chemical and enzymatic gel sequencing technics using 3′ and 5′ end-labeled RNA. This RNA is very different from 5S rRNA of the cellular slime mold Dictyostelium discoideum (36 nucleotides are different), and shows greater similarity to 5S rRNM from Protozoa and Metazoa than to those from fungi.