High‐resolution, multicontrast three‐dimensional‐MRI characterizes atherosclerotic plaque composition in ApoE–/– mice ex vivo
Open Access
- 19 November 2004
- journal article
- research article
- Published by Wiley in Journal of Magnetic Resonance Imaging
- Vol. 20 (6), 981-989
- https://doi.org/10.1002/jmri.20211
Abstract
Purpose To systematically investigate intrinsic MR contrast mechanisms that would facilitate plaque characterization and quantification in the aortic root and brachiocephalic artery of ApoE–/– mice ex vivo. Materials and Methods To establish unambiguous MR parameters for routinely analyzing atherosclerotic plaque ex vivo at 11.7 T, relaxation times of plaque components were quantitatively assessed. Magnetization transfer and lipid‐proton three‐dimensional MR imaging was investigated for visualization of collagen‐ and lipid‐rich plaque regions, respectively. A three‐dimensional multiecho sequence with a spatial resolution of 47 × 47 × 63 μm was implemented providing a variable degree of T2‐weighting. Results Relaxation time measurements showed clear tissue heterogeneity between atherosclerotic plaque components in the T2‐values, but similar T1‐values at 11.7 T (T1/T2 mean ± SD; cellular plaque component: 1.2 ± 0.3 seconds/26.3 ± 0.4 msec; fibrofatty plaque component: 1.1 ± 0.2 seconds/13.7 ± 2.0 msec). The three‐dimensional multiecho sequence allowed the calculation of the intrinsic proton density and T2‐maps. The sum of the multiecho data provided strong T2‐weighting that facilitated quantification of various components of atherosclerotic plaque in the mouse aortic root and correlated well with histology (P < 0.0001). Conclusion High‐resolution MRI allows for accurate classification and quantification of atherosclerotic plaque components in the aortic root of mice. J. Magn. Reson. Imaging 2004;20:981–989.Keywords
This publication has 40 references indexed in Scilit:
- High‐resolution MRI with cardiac and respiratory gating allows for accurate in vivo atherosclerotic plaque visualization in the murine aortic archMagnetic Resonance in Medicine, 2003
- Characterization of aortic root atherosclerosis in ApoE knockout mice: High‐resolution in vivo and ex vivo MRM with histological correlationMagnetic Resonance in Medicine, 2003
- Repeated Three-Dimensional Magnetic Resonance Imaging of Atherosclerosis Development in Innominate Arteries of Low-Density Lipoprotein Receptor-Knockout MiceCirculation, 2002
- Lessons From Sudden Coronary DeathArteriosclerosis, Thrombosis, and Vascular Biology, 2000
- A Definition of Advanced Types of Atherosclerotic Lesions and a Histological Classification of AtherosclerosisArteriosclerosis, Thrombosis, and Vascular Biology, 1995
- Parameter relations for the Shinnar-Le Roux selective excitation pulse design algorithm (NMR imaging)IEEE Transactions on Medical Imaging, 1991
- High‐resolution H NMR spectral signature from human atheromaMagnetic Resonance in Medicine, 1988
- High-resolution proton NMR spectra of human arterial plaqueBiochemical and Biophysical Research Communications, 1987
- A carbon-13 NMR study of aortic lesions and cholesteryl ester rich lipoproteins from atherosclerotic rabbitsBiochemistry, 1982
- Compensation for pulse imperfections in NMR spin-echo experimentsJournal of Magnetic Resonance (1969), 1981