Isolation and characterization of Methanobacterium uliginosum sp. nov. from a marshy soil

Abstract

A rod-shaped Gram-positive methanogenic bacterium was isolated from marshy soil. It uses H₂ – CO₂ as sole energy and carbon source. Optimal growth occurs around 40 °C. The cell wall contains pseudomurein. The G + C content of the DNA is about 32 mol%. The DNA – DNA homology values of the isolate with Methanobacterium bryantii strains M.o.H. and M.o.H.-G. are 53 and 7%, respectively. The isolate is considered to be a new species within the genus Methanobacterium and it was named Methanobacterium uliginosum.