MEDIUM FOR DIFFERENTIATING THE GRAM-NEGATIVE, NONFERMENTING BACILLI OF MEDICAL INTEREST

Abstract

An agar-slant medium is described for differentiating gram-negative, nonfermenting bacilli of medical interest. Differences in the ability of bacilli to grow anaerobically in the presence of nitrate, to produce pH changes, to produce N2 from nitrite and nitrate (singly and in combination), to fluoresce and to oxidize a drop of glucose solution to acid in the presence of high peptone concentrations were used in the development of the medium. Organisms differentiated by the medium include Pseudomonas aeruginosa, Bacterium anitratum, Mima polymorpha and Vibrio alcaligenes or Alcaligenes faecalis.