Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II
- 1 April 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (4), 761-764
- https://doi.org/10.1073/pnas.68.4.761
Abstract
The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.Keywords
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