Analysis of filamin and α‐actinin binding to actin by the stopped flow method
- 28 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (3), 408-410
- https://doi.org/10.1016/0014-5793(93)80847-n
Abstract
We ascertained by the stopped flow method the overall association rate constant, k +1, of filamin and α-actinin to fluorescently labelled filamentous actin of ~ 1.3 × 106M−1 · s−1 and ~ 1.0 × 106M−1 · s−1 as well as the overall dissociation rate constant,k −1 of ~ 0.6s−1 and ~ 0.4s−1, respectively. The overall equilibrium constant, K, for filamin and α-actinin to actin deduced from the relation K = agree well with published data.
Keywords
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