Isolation of a type C RNA virus from an established human histiocytic lymphoma cell line.

Abstract

A type C RNA virus was detected in the culture fluids of the SU-DHL-1 human histiocytic lymphoma cell line. In electron micrographs, the virus closely resembled other typical mammalian type C RNA tumor viruses in size and morphology. Viral RNA-dependent DNA polymerase activity was demonstrated in particles (densities of 1.15 and 1.22 g/ml) in the microsomal cytoplasmic fraction and in pellets of culture fluids. The enzyme is partially inhibited by antibodies to the RNA-dependent DNA polymerases of simian sarcoma virus and RD-114 virus but not by antibody to the polymerase of murine leukemia virus, suggesting some degree of relatedness to type C virus of subhuman primate origin. Typical syncytial microplaques were induced when SU-DHL-1 cells were cocultivated with rat XC cells. Although no focus formation was noted in similarly cocultivated mouse UC1-B cell cultures, the numbers of foci induced in rat embryo fibroblasts by murine sarcoma virus were significantly increased by coinfection with the virus from SU-DHL-1 cell culture fluids. No other evidence of infectivity, inducibility or capacity for helper rescue of defective murine sarcoma virus genomes were detected to date in cocultivation studies with a spectrum of ribroblastic and other nonlymphoid indicator cell lines of human and other species of origin.