Feulgen Staining of Rabbit Spermatozoa: Hydrolysis Time and Distribution of Chromatin
- 1 January 1965
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 40 (4), 193-197
- https://doi.org/10.3109/10520296509116406
Abstract
A period of 12–16 min was found to be optimum for hydrolysis, in 1 N HCl at 60 C, of rabbit spermatozoa for quantitative Feulgen staining. The gradient of optical density along the longitudinal axis of Feulgen-stained rabbit sperm cell nuclei followed a typical pattern, deviating from a linear regression. A 2 μ diameter spot centered at the midpoint of the nucleus with uniform density approximating the mean density was present in most of the cells measured. This central spot can be used with reliance in DNA assays using light beam core sampling methods of microspectrophotometry.This publication has 5 references indexed in Scilit:
- Optical Density Profiles in Feulgen-Stained Bovine Spermatozoan NucleiJournal of Dairy Science, 1964
- Feulgen-DNA Cytophotometry of Bovine SpermatozoaJournal of Dairy Science, 1964
- Effects of Age of Stain, Hydrolysis Time, and Freezing of the Cells on the Feulgen-DNA Content of Bovine SpermatozoaJournal of Dairy Science, 1964
- A cytochemical study of the feulgen nucleal reactionChromosoma, 1950
- Mikroskopisch-chemischer Nachweis einer Nucleinsäure vom Typus der Thymonucleinsäure und die- darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten.Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1924