Dependence of Human Stem Cell Engraftment and Repopulation of NOD/SCID Mice on CXCR4
- 5 February 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 283 (5403), 845-848
- https://doi.org/10.1126/science.283.5403.845
Abstract
Stem cell homing and repopulation are not well understood. The chemokine stromal cell–derived factor-1 (SDF-1) and its receptor CXCR4 were found to be critical for murine bone marrow engraftment by human severe combined immunodeficient (SCID) repopulating stem cells. Treatment of human cells with antibodies to CXCR4 prevented engraftment. In vitro CXCR4-dependent migration to SDF-1 of CD34+CD38−/low cells correlated with in vivo engraftment and stem cell function. Stem cell factor and interleukin-6 induced CXCR4 expression on CD34+ cells, which potentiated migration to SDF-1 and engraftment in primary and secondary transplanted mice. Thus, up-regulation of CXCR4 expression may be useful for improving engraftment of repopulating stem cells in clinical transplantation.Keywords
This publication has 20 references indexed in Scilit:
- Function of the chemokine receptor CXCR4 in haematopoiesis and in cerebellar developmentNature, 1998
- Disturbed CD4+ T Cell Homeostasis and In Vitro HIV-1 Susceptibility in Transgenic Mice Expressing T Cell Line–tropic HIV-1 ReceptorsThe Journal of Experimental Medicine, 1998
- Phorbol Esters and SDF-1 Induce Rapid Endocytosis and Down Modulation of the Chemokine Receptor CXCR4The Journal of cell biology, 1997
- The Chemokine SDF-1 Is a Chemoattractant for Human CD34+ Hematopoietic Progenitor Cells and Provides a New Mechanism to Explain the Mobilization of CD34+ Progenitors to Peripheral BloodThe Journal of Experimental Medicine, 1997
- Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1Nature, 1996
- The Biology of Hematopoietic Stem CellsAnnual Review of Cell and Developmental Biology, 1995
- Structure and Chromosomal Localization of the Human Stromal Cell-Derived Factor 1 (SDF1) GeneGenomics, 1995
- Quantitation, Mobilization, and Clinical Use of Long-Term Culture-Initiating Cells in Blood Cell AutograftsJournal of Hematotherapy, 1995
- Cytokine Stimulation of Multilineage Hematopoiesis from Immature Human Cells Engrafted in SCID MiceScience, 1992
- The SCID-hu Mouse: Murine Model for the Analysis of Human Hematolymphoid Differentiation and FunctionScience, 1988