Studies on extracellular ribonucleases of Ustilago sphaerogena. Purification and properties
- 1 February 1968
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 106 (3), 601-607
- https://doi.org/10.1042/bj1060601
Abstract
Four ribonucleases were isolated from culture media of Ustilago sphaerogena. They were designated ribonucleases U1, U2, U3 and U4. They were purified about 1600-, 3700-, 1100- and 16-fold respectively. It was shown by gel filtration that ribonucleases U1, U2 and U3 have molecular weights about 10000 like ribonuclease T1, and that ribonu-clease U4 is much larger. Ribonucleases Ul, U2 and U3 are thermostable, but ribonuclease U4 is not. The pH optimum of ribonucleases U1 and U4 is pH 8.0-8.5, and that of ribonucleases U2 and U3 is pH 4.5.This publication has 6 references indexed in Scilit:
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