Expression of IFN A genes in subpopulations of peripheral blood cells
- 1 April 1994
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 86 (4), 717-725
- https://doi.org/10.1111/j.1365-2141.1994.tb04820.x
Abstract
The polymerase chain reaction (PCR) was used to detect the expression of IFN A genes in general (with 'universal' primers) and specifically the expression of mRNA transcripts encoding the subtypes IFN-alpha-1, -alpha-2, -alpha-4, -alpha-5 and -alpha-14 (with gene specific primers) in normal human peripheral blood mononuclear cells (PBMC) and PBMC subpopulations. Our examination revealed that all transcripts tested for could be detected not only following induction with inducers such as Sendai virus, Semliki Forest virus and poly(I):poly(C), but also in the absence of induction. IFN A1, IFN A2 and IFN A4 mRNAs were found to constitute the major transcripts of Sendai virus and poly(I):poly(C) induced PBMC. Fractionation of PBMC into T cells, B cells, adherent cells, mononuclear (MN) cells and polymorphonuclear (PMN) cells revealed that these cell populations all contain specific IFN A mRNA transcripts both in the absence of an inducer and following induction with Sendai virus. The proportion of IFN A transcripts detected was dependent on the cell type investigated. IFN A1, IFN A2 and IFN A4 transcripts constituted the major RNA species present in PBMC and PMN cells. In MN cells IFN A5 transcripts were also present as a major IFN RNA species. Expression of the IFN A transcripts tested for in T cells, B cells and adherent cells did not vary significantly. These results emphasize the importance of identifying IFN A subtype expression in order to further our understanding of the biological significance of differential regulation and expression of particular IFN-alpha subtypes.Keywords
This publication has 29 references indexed in Scilit:
- Post‐transcriptional regulation of interferon‐alpha 4 subtype production by lymphoblastoid cellsHematological Oncology, 1993
- Interferon-alpha but not -beta Genes Require De Novo Protein Synthesis for Efficient Expression in Human MonocytesScandinavian Journal of Immunology, 1992
- Flow Cytometric Analysis of Natural Interferon‐α Producing CellsScandinavian Journal of Immunology, 1991
- Infrequent but Efficient Interferon-α-Producing Human Mononuclear Leukocytes Induced by Herpes Simplex Virus In Vitro Studied by Immuno-Plaque and Limiting Dilution AssaysJournal of Interferon Research, 1990
- Characterization of the Blood Mononuclear Leucocytes Producing Alpha Interferon after Stimulation with Herpes Simplex Virus in Vitro, by Means of Combined Immunohistochemical Staining and in Situ RNA‐RNA HybridizationScandinavian Journal of Immunology, 1989
- Determination of Herpes Simplex Virus-Induced Alpha Interferon-Secreting Human Blood Leucocytes by a Filter Immuno-Plaque AssayScandinavian Journal of Immunology, 1988
- Human Monocytes and Lymphocytes Produce Different Mixtures of α-Interferon SubtypesJournal of Interferon Research, 1986
- Priming of leukocytes selectively increases the level of some interferon-α subtypes and not othersBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Properties of human natural interferon‐producing cells stimulated by tumor cell linesEuropean Journal of Immunology, 1983
- Human peripheral null lymphocytes II. Producers of type‐1 interferon upon stimulation with tumor cells, Herpes simplex virus and Corynebacterium parvumEuropean Journal of Immunology, 1980