‘Small’-proteoglycan: collagen interactions: Keratan sulphate proteoglycan associates with rabbit corneal collagen fibrils at the ‘a’ and ‘c’ bands
- 1 September 1985
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 5 (9), 765-774
- https://doi.org/10.1007/bf01119875
Abstract
L. Proteoglycans (PGs) in rabbit corneal stroma and mouse sclera have been stained for electron microscopy with Cupromeronic blue in a critical electrolyte concentration (CEC) mode, with and without prior digestion of the tissue by keratanase or chondroitinase ABC to remove the keratan sulphate (KS) or chondroitin-dermatan sulphates (CS or DS) respectively. 2. Two classes of PGs, located orthogonally to the corneal collagen fibrils at either the ‘step’ (band ‘a’ or ‘c’) or gap zone (band ‘d’ or ‘e’) are shown to be KS-PGs or DS-PGs respectively. Four separate and specific PG binding sites on Type I collagen fibrils have thus been identified. 3. Rabbit corneal KS and DS PGs each contain two kinds of PG (Gregory JD, Coster L & Damle SP (1982) J. Biol. Chem. 257 , 6965–6970). We propose that each ‘small’ protein-rich PG is associated with a specific binding site on the collagen fibril.This publication has 21 references indexed in Scilit:
- The periphery of the developing collagen fibril. Quantitative relationships with dermatan sulphate and other surface-associated speciesBiochemical Journal, 1984
- Bovine Tracheal Cartilage Proteoglycans. Variations in Structure and Composition with AgeCollagen and Related Research, 1983
- Proteoglycans of bovine periodontal ligament and skin. Occurrence of different hybrid-sulphated galactosaminoglycans in distinct proteoglycansBiochemical Journal, 1982
- Dermatan sulphate-rich proteoglycan associates with rat tail-tendon collagen at the d band in the gap regionBiochemical Journal, 1981
- Proteoglycan-collagen arrangements in developing rat tail tendon. An electron microscopical and biochemical investigationBiochemical Journal, 1981
- Collagen–proteoglycan interactions. Localization of proteoglycans in tendon by electron microscopyBiochemical Journal, 1980
- Observations on the collagen and proteinpolysaccharide complex of rabbit corneal stromaJournal of Cell Science, 1969
- Distribution of Acid Glycosaminoglycans in Human Articular CartilageNature, 1967
- Observations on the acid glycosaminoglycan (mucopolysaccharide) content of the matrix of aging cartilage.Annals Of The Rheumatic Diseases, 1965
- The fine structure of developing bone in the embryonic fowlProceedings of the Royal Society of London. B. Biological Sciences, 1957