Cytopathic and Non-cytopathic Biotypes of Border Disease Virus Induce Polypeptides of Different Molecular Weight with Common Antigenic Determinants

Abstract

Ten monoclonal antibodies have been raised against lysates of cells infected with cytopathic border disease virus (BDV). These antibodies all recognize noncytopathic BDV and react with a number of different strains of bovine viral diarrhoea virus (BVDV). Studies with radiolabelled cell lysates show that all the antibodies precipitate two polypeptides of apparent Mr 80000 and 130000 from cells infected with cytopathic virus and a single polypeptide of apparent Mr 120000 from cells infected with non-cytopathic virus. Two of the monoclonal antibodies react on immunoblots and show the same pattern of reactivity indicating that these three polypeptides are antigenically related.