The Structure of Human‐Plasma Low‐Density Lipoprotein B
- 1 January 1978
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 82 (1), 73-90
- https://doi.org/10.1111/j.1432-1033.1978.tb11998.x
Abstract
1. X-ray small-angle scattering of human plasma lipoprotein B of the low-density fraction (rho = 1.016--1.060 g.cm-3) has been recorded to high precision at different electron density contrasts. 2. The overall structure of the particles is characterized by a quasi-spherical shape and radial symmetry. A maximum diameter of 23 nm and a molecular weight of 2.4 X 10(6) have been determined. 3. The internal structure is described in terms of a model consisting of spherical layers with different electron densities indicating that the neutral lipids are arranged in the core of the molecule up to a radius of about 8 nm surrounded by a monolayer of free cholesterol, phospholipids and protein. The neural lipids are shown to be in an ordered, liquid crystalline state at 4 degrees C and to undergo a thermotropic transition into a disordered state at higher temperatures.Keywords
This publication has 49 references indexed in Scilit:
- A new method for the evaluation of small-angle scattering dataJournal of Applied Crystallography, 1977
- ErratumJournal of Molecular Biology, 1976
- Structure of human serum lipoproteins in solution: II. Small-angle X-ray scattering study of HDL3 and LDLJournal of Molecular Biology, 1976
- Structure of human serum lipoproteins in solution: I. Theory and techniques of an X-ray scattering approach using solvents of variable densityJournal of Molecular Biology, 1976
- NMR studies of pig low- and high-density serum lipoproteins: Molecular motions and morphologyBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
- The structure of a high density lipoprotein (HDL 3 ) from porcine plasmaProceedings of the Royal Society of London. B. Biological Sciences, 1974
- X‐ray small angle scattering of human plasma high density lipoprotein LpA from HDL2: Application of a new evaluation methodFEBS Letters, 1974
- Small angle X‐ray scattering of LpA, the major lipoprotein family of human plasma high density lipoprotein HDL3FEBS Letters, 1972
- On the structure of human serum low density lipoproteinJournal of Molecular Biology, 1972
- Application of spline functions to the correction of resolution errors in small-angle scatteringJournal of Applied Crystallography, 1971