A Small Gold-Conjugated Antibody Label: Improved Resolution for Electron Microscopy
- 24 April 1987
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 236 (4800), 450-453
- https://doi.org/10.1126/science.3563522
Abstract
A general method has been developed to make the smallest gold-conjugated antibody label yet developed for electron microscopy. It should have wide application in domainal mapping of single molecules or in pinpointing specific molecules, sites, or sequences in supramolecular complexes. It permits electron microscopic visualization of single antigen-binding antibody fragments (Fab') by covalently linking an 11-atom gold cluster to a specific residue on each Fab' such that the antigenic specificity and capacity are preserved. The distance of the gold cluster from the antigen is a maximum of 5.0 nanometers when the undecagold-Fab' probe is used as an immunolabel.Keywords
This publication has 16 references indexed in Scilit:
- MASS MAPPING WITH THE SCANNING TRANSMISSION ELECTRON MICROSCOPEAnnual Review of Biophysics, 1986
- Undecagold clusters for site-specific labeling of biological macromolecules: simplified preparation and model applicationsJournal of Inorganic Biochemistry, 1986
- Synthesis of undecagold cluster molecules as biochemical labeling reagents. 1. Monoacyl- and mono[N-(succinimidooxy)succinyl]undecagold clustersBiochemistry, 1984
- Undecagold labeling of a glycoprotein: STEM visualization of an undecagoldphosphine cluster labeling the carbohydrate sites of human haptoglobin—hemoglobin complexJournal of Ultrastructure Research, 1983
- Biospecific Labeling with Undecagold: Visualization of the Biotin-Binding Site on AvidinScience, 1982
- Identification and mass analysis of human fibrinogen molecules and their domains by scanning transmission electron microscopyJournal of Molecular Biology, 1981
- Staphylococcal protein a bound to colloidal gold: A useful reagent to label antigen-antibody sites in electron microscopyImmunochemistry, 1977
- Crystallographic structure studies of an IgG molecule and an Fc fragmentNature, 1976
- Electron microscopic localization of two proteins on the surface of the 50 S ribosomal subunit of Escherichia coli using specific antibody markersJournal of Molecular Biology, 1974
- Communication to the editors: An immunocolloid method for the electron microscopeImmunochemistry, 1971