A Simple and Effective Method for Homolysis with a Hypoxanthine-Xanthine Oxidase System and Alteration of Erythrocyte Phospholipid Composition during the Hemolysis

Publisher Website
Google Scholar
Abstract
A very rapid hemolysis was found to be caused by active oxygen species produced by a hypoxanthine-anthine oxidase system with very low concentrations of hypoxanthine. The addition of superoxide dismutase or catalase inhibited the hemolysis, indicating that O 2− and H 2 O 2 participate in this system. The extent of erythrocyte hemolysis was found to depend on the sex of the human donor. The change in phospholipid composition before and after hemolysis in human erythrocytes from donors of each sex was compared by thin layer chromatography. A significant decrease in phosphatidylethanolamine content and a concomitant increase in altered phospholipid fraction were observed in erythrocytes from male donors, suggesting that these erythrocytes were easily attacked by active oxygen species to produce modified phosphatidylethanolamine.