Preferential Binding of RNA Polymerase to the Thymidylic Acid-rich Fragments Obtained from Bacteriophage f1 DNA

Abstract

The interaction between the T-rich fragments obtained from bacteriophage f1 DNA and RNA polymerase (EC 2. 7. 7. 6) was studied. The RNA polumerase bound more abundasntly to the T-rich fragments than to the mean f1 DNA fragments having almost equal molecular size. When made to react with RNA polymerase, the RNA synthesis proceeded twice as fast on the T-rich fragments as on the mean f1 DNA fragments. One datum suggested that the information on the T-rich fragments could be transcribed repeatedly in vitro by the RNA polymerase.