Improved Preparation and Cooperative Calcium Contraction of Glycerinated Vorticella1
- 1 March 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 83 (3), 795-798
- https://doi.org/10.1093/oxfordjournals.jbchem.a131974
Abstract
An improved method for the preparation of glycerinated Vorticella convallaria was investigated. The pretreatment of living vorticellas with a medium containing 0.1% saponins and subsequent treatment with an extraction medium containing 35% glycerol at about 0°C was satisfactory. The equilibrium average length of contractile stalks of glycerinated vorticellas was measured at various free calcium concentrations in the reaction medium. It was found that the contractile element in the spasmoneme of the stalk is contracted by a cooperative interaction involving at least two calcium ions.This publication has 2 references indexed in Scilit:
- The involvement of pH, Adenosine triphosphate, calcium, and magnesium in the contraction of the glycerinated stalks of VorticellaJournal of Cellular and Comparative Physiology, 1965
- Der mechanismus eines neuen, von der muskelkontraktion verschiedenen kontraktionszyklusBiochimica et Biophysica Acta, 1958