Nucleoside 3'-phosphotriesters as key intermediates for the oligoribonucleotide synthesis. IV. New method for removal of 2,2,2-trichioroethyl group and31NMR as a new tool for analysis of deblocking of intemucleotide phosphate protecting groups
Open Access
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (7), 2321-2330
- https://doi.org/10.1093/nar/4.7.2321
Abstract
Zinc/acetylacetone/pyridine treatment has been designed as a very efficient method for removal of 2,2,2-trichloroethyl group from phosphoesters. Internucleotide and terminal 2,2,2-trichloroethyl phosphotriesters were transformed to corresponding diesters quantitatively. Much less reactive 2,2,2-trichloroethyl phosphodiesters produced monoesters with Ca. 90% yield.Keywords
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