Nuclear matrix-bound DNA synthesis: an in vitro system

Abstract

A nuclear matrix-bound in vitro replicational system that utilizes .alpha.-polymerase and matrix-attached DNA was isolated from regenerating rat liver and characterized for in vitro requirements. .beta.-Polymerase is a minor component of the matrix in vitro system. Evidence is presented for the native association of .alpha.-polymerase with the nuclear matrix and for the parallel induction of matrix-bound .alpha.-polymerase and in vivo DNA synthesis during the proliferative response following partial hepatectomy. The attachment of .alpha.-polymerase to the matrix may be an important step in the assembly of functional replisomes. Prereplicative and late-replicative rises in nuclear and matrix-bound .beta.-polymerase activity are demonstrated and these results are discussed in terms of possible genome screening.