High-Resolution Microradiography Using Low-Voltage X-Rays

Abstract

Mathematical considerations of the concept of extremely high resolution in microradiography have allowed the derivation of optimal conditions for studies of biological material. Specimen thickness must be kept below 4 microns, and as a consequence very low voltages over the x‐ray tube are needed. Using a special method for preparing the specimen for analysis, it has been possible to obtain microradiograms at x‐ray potentials of a few hundred volts. The absolute resolution of the x‐ray image is comparable to that of the light microscope.