Formation of the COOH‐terminal amide group of thyrotropin‐releasing‐factor

Abstract
The precursors of peptide hormones that possess a COOH‐terminal α‐amide group contain a glycine residue following the amino acid which is amidated in the hormone. 〈Glu—His—Pro—Gly was synthesized as a putative precursor of thyroliberin. Bovine pituitary neurosecretory granules were shown to contain an amide group‐forming activity associated with an M r of about 62000 protein(s) which converts the tetrapeptide to thyroliberin.