DNA Polymerase of Chicken Embryo: Purification and Properties
- 1 July 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (7), 1781-1785
- https://doi.org/10.1073/pnas.69.7.1781
Abstract
The almost complete purification of the previously reported DNA polymerase of chicken embryo is described; in the final isolation step the enrichment of the enzyme, which comprises about 0.01% of the protein extractable from 10-day-old embryos, is more than 9000-fold. The enzyme is a basic protein (pI = 9.15), of molecular weight about 27,000. It exhibits no nuclease activity, requires Mn(2+) ion for activity, and may contain Zn. It is very sensitive to oxidation.Keywords
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