CALCULATION OF RADIOIMMUNOCHEMICAL DETERMINATIONS BY SPLINE-APPROXIMATION

Abstract

A simplified method, based on the spline approximation, is reported for the calculation of the standard curves of radioimmunochemical determinations. The mathematical function can be manipulated with a pocket calculator, thus making it available for a large number of users. Optimal quality control can be achieved in the preparation of the standard curves and in the interpolation of unknown plasma samples. The recalculation of interpolated values form their own standard curve revealed an error of 4.9% which would normally be an error of interpolation. The new method was compared with 2 established methods for 8 different radioimmunochemical determinations. The measured values of the standard curve showed a weighting, and there was a resulting quality control of these values, which, according to their statistical evaluation, were more accurate than those of other models. In contrast with other models, the described method makes no mathematical or kinetic preconditions with respect to the dose-response relationship. To achieve optimal reaction conditions, experimentally determined reaction data are preferable to model theories.