Cell cycle dependent changes in potassium transport
- 1 September 1976
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 89 (1), 123-132
- https://doi.org/10.1002/jcp.1040890112
Abstract
K transport has been investigated during progression of cultured Ehrlich ascites tumor cells through the cell cycle. Using a double thymidine block technique, Ehrlich cells carried in continuous culture have been synchronized, as verified by simultaneous monitoring of cell number, cell volume, 3H‐thymidine incorporation and mitotic index. Unidirectional influx, efflux and cell content of K have been monitored throughout the cell cycle. The nature of the pump mediated, ouabain‐sensitive K flux and the furosemide‐sensitive component of K flux, presumably representing K‐K exchange, have also been evaluated. In early S period the ouabain sensitive component, representing the Na‐K pump, comprises 52% of the total unidirectional K influx. During S period the pump activity increases to a maximum of 65% of the unidirectional K influx and subsequently declines during G2 period to a minimum of 40% in mid G2. During M and early S the activity again rises. As the ouabain sensitive component becomes maximal in late S period, the furosemide sensitive component diminishes from approximately 30% of the total influx to approximately 10%. The same pattern is observed in the G2 period. As the pump component diminishes, the furosemide sensitive component increases. Furosemide sensitive K efflux has also been monitored and the pattern is equivalent to that observed in the influx studies. No change in net K flux is observed in the presence of furosemide. This indicates that the furosemide sensitive component represents an exchange component for K. These results are consistent with the conclusion that the alterations in exchange and pump fluxes are physiological events associated with progression of the cell cycle.This publication has 22 references indexed in Scilit:
- Relationship between intracellular K+ concentrations and K+ fluxes in growing and contact-inhibited cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- The effects of ouabain on the cell mitotic cycle of mouse lymphoblastsJournal of Cellular Physiology, 1975
- CYCLIC CHANGES IN THE CELL SURFACEThe Journal of cell biology, 1974
- Sequence of Events in Plasma Membrane Assembly during the Cell CycleNature, 1973
- A Unifying Hypothesis Concerning the Nature of Malignant GrowthProceedings of the National Academy of Sciences, 1972
- Na-linked Transport of Organic SolutesPublished by Springer Nature ,1972
- The behaviour of the sodium pump in red cells in the absence of external potassiumThe Journal of Physiology, 1967
- Cation Metabolism in Relation to Cell Size in Synchronously Grown Tissue Culture CellThe Journal of general physiology, 1967
- CYTOKINETIC ANALYSIS OF TUMOR GROWTHProceedings of the National Academy of Sciences, 1966
- POTASSIUM AND SODIUM MOVEMENTS IN THE EHRLICH MOUSE ASCITES TUMOR CELLThe Journal of general physiology, 1958