Assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera
- 1 March 1981
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 31 (3), 1062-1070
- https://doi.org/10.1128/iai.31.3.1062-1070.1981
Abstract
A reliable and reproducible method for determining specific reactivity to herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) in human sera has been developed. Human sera were used to immunoprecipitate HSV-specific glycoprotein antigens from both HSV-1- and HSV-2-infected cell extracts. The viral glycoproteins precipitated from these extracts were then analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to detect specific reactivity of the sera with distinct type-specific antigens of HSV-1, HSV-2, or both as well as with type-common glycoprotein antigens. By examining a large number of human sera, this method was found to be more reliable than the standard microneutralization test in discriminating between single-positive (positive for HSV-1 or HSV-2) and double-positive (positive for HSV-1 and HSV-2) sera.This publication has 30 references indexed in Scilit:
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