Simultaneous Determination of Codeine and Morphine in Biological Samples by Gas Chromatography with Electron Capture Detection
- 1 September 1977
- journal article
- research article
- Published by Wiley in Acta Pharmacologica et Toxicologica
- Vol. 41 (3), 273-279
- https://doi.org/10.1111/j.1600-0773.1977.tb02148.x
Abstract
A sensitive gas chromatographic method for simultaneous determination of codeine and morphine in plasma and brain samples is described. It involves solvent extraction of compounds from plasma, derivatization with pentafluoropropionic anhydride and subsequent separation on a 3% OV-17 column. Quantification is performed with electron capture detection. Sensitivity of the method (0.75 ng of morphine and 7.5 ng of codeine in a sample) makes it especially useful for pharmacokinetic investigations. It was successfully applied to determine the time course of codeine and its metabolite morphine after i.v. codeine administration to the rat.Keywords
This publication has 8 references indexed in Scilit:
- Rapid g.l.c. method for the separation of picogram quantities of morphine and codeineJournal of Pharmacy and Pharmacology, 1976
- Quantitative GLC Determination of Codeine in PlasmaJournal of Pharmaceutical Sciences, 1976
- Gas-Chromatographic Measurement of Codeine and Norcodeine in Human PlasmaClinical Chemistry, 1975
- A rapid g.l.c. procedure for the determination of codeine and norcodeine in biological fluids based on micro-phase extraction techniquesJournal of Pharmacy and Pharmacology, 1975
- Determination of morphine and codeine in post-mortem specimensAnalytical Chemistry, 1975
- Quantitative determination of morphine in biological samples by gas-liquid chromatography and electron-capture detectionJournal of Pharmacy and Pharmacology, 1975
- Determination of morphine in biologic fluids by electron capture gas-liquid chromatographyAnalytical Chemistry, 1974
- Gas Chromatographic Determination of Codeine in Serum and UrineJournal of Pharmaceutical Sciences, 1966