THE ISOLATION OF T4-SPECIFIC RNA ON A DNA-CELLULOSE COLUMN
- 1 March 1962
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 48 (3), 400-408
- https://doi.org/10.1073/pnas.48.3.400
Abstract
T4- specific RNA has been quantitatively separated from E. coli RNA by chromatography on columns made of T4 DNA linked to cellulose. Apparently, the basis of the fractionation is specific hydrogen-bond formation between the DNA of the column and RNA molecules complementary in base sequence. A DNA-cellulose column containing DNA from the T4 deletion mutant r1272 has been used to purify a type of RNA which appears to be specific for the rII region of the phage genome.Keywords
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