Isolation and nucleotide sequence of the extracellular acid protease gene (ACP) from the yeast Candida tropicalis

Abstract

The extracellular acid protease of Candida tropicalis was purified from the supernatant fraction of culture medium containing bovine serum albumin as nitrogen source and the NH2-terminal amino acid (aa) sequence of the protein was determined. The gene for the acid protease (ACP) was isolated using a pool of synthetic oligonucleotides as a probe and a segment of the deduced aa sequence was found to be in agreement with the NH2-terminal aa sequence of the protein. The deduced aa sequence of ACP is similar to the aa sequence of proteases of the pepsin family. The nucleotide sequence of the 5′ portion of this gene revealed a coding sequence for a 60 residue propeptide containing two Lys—Arg amino acid pairs that have been identified as sites for peptidase processing of several exported peptides and proteins. The final Lys—Arg site occurs at the junction with the mature extracellular form or the acid protease