The nucleotide sequence of tyrosine tRNA from Bacillus stearothermophilus

Abstract

The nucleotide sequence of tRNA^TYr from B. stearothermophilus has been determined: pG-G-A-G-G-G-G-S⁴U-A-G-C-G-A-A-G-U-Gm-G-C-U-A-A-m¹A-C-G-C-G-G-C-G-G-A-C-U-Q-U-A-ms²i⁶A-A-Ψ-C-C-G-C-U-C-C-C-U-U-U-G-G-C-G-G-T-Ψ-C-G-A-A-U-C-C-G-U-C-C-C-C-C-U-C-C-A-C-C-A_OH. A combination of classical fingerprinting methods, partial nuclease P₁ digestion and two-dimensional homochromatography and a rapid “read off” sequencing gel technique were used to establish the complete nucleotide sequence.