Impaired Motility and Random Migration of Viatl Polymorphonuclears in Vitro after therapy with Oral Aromatic Retionoid in Psoriasis

Abstract

Vital polymorphonuclears of healthy person and of patients with psoriasis before and after therapy with oral rettnoid (Ro 10–9359) were observed in vitro under standradized conditions. In this model normal pllymorphonuclears showed well‐defined changes entering (A) a stage of resting and accommondation, (B) a state of Movement in loco, and finally, (C) a stage of miration. The rectilinear migration of cells was accompanied bycharacteristic movements of the cytoplasmic granula towards the cytocenturm. Just before the onset of migration, the cell organelles ordered themselves in a defined position within cytoplasm. The survival time of the cells was about 48 haurs, and their degeneration in vitro was reproducible. Polymorphonuclears of psoriasis patients without respiration before therapy with oral retionid showed no changes. In Contrast, the in vitro motility of polymorphonuclears of psoriasis under oral retiniod therapy showed distinct alterations: the movement of the cytoplasmic granula was strongly decelerated, and the regualr arrangement of the cell organelles did not appear. The function of the cytocentrum was obviously impaired; the cells persisted longer in the resting stage and reached the movement state later. A stage of migration failer to appear. The survival time was reduced to approximately 24 hours. These observations suggest that the oral retinoid interferes with the microtubular cell system and inhibits the directional migration of the polymorphonuclear in vitro.