Enzymatic RNA amplification of the enteroviruses
- 1 March 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 28 (3), 438-442
- https://doi.org/10.1128/jcm.28.3.438-442.1990
Abstract
Enteroviruses are among the most common causes of childhood infection. Current diagnostic techniques are often too slow and too insensitive to benefit the patient optimally. This report describes a modified polymerase chain reaction technique by which enteroviral RNA can be amplified, over a few hours, to a level detectable by agarose mini-gel electrophoresis or nucleic acid hybridization or both. Three oligomeric regions of great homology among the enteroviruses were identified and designated as a potential primer pair and probe. With this combination, all 11 of the enterovirus serotypes tested, representing the major subgroups of these pathogens, were successfully amplified and detected. The sensitivity and rapidity of this new assay speak to its potential clinical applicability in the diagnosis of enterovirus infections.This publication has 25 references indexed in Scilit:
- Development and application of RNA probes for the study of picornavirusesMolecular and Cellular Probes, 1988
- RNA target loss during solid phase hybridization of body fluids—a quantitative studyMolecular and Cellular Probes, 1987
- The Complete Nucleotide Sequence of Coxsackievirus B4 and Its Comparison to Other Members of the PicornaviridaeJournal of General Virology, 1987
- Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotidesBiochemical and Biophysical Research Communications, 1987
- Genome of coxsackievirus B3Virology, 1987
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- In vitro activity of WIN 51711, a new broad-spectrum antipicornavirus drugAntimicrobial Agents and Chemotherapy, 1985
- Complete nucleotide sequences of all three poliovirus serotype genomesJournal of Molecular Biology, 1984
- Echovirus Type 7 Meningitis in Young ChildrenArchives of Pediatrics & Adolescent Medicine, 1981
- Enzyme-Linked immunosorbent assay for the detection and identification of coxsackie b antigen in tissue cultures and clinical specimensJournal of Medical Virology, 1980