A protein complex mediating mRNA degradation in Escherichia coli
- 1 November 1994
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 14 (4), 717-729
- https://doi.org/10.1111/j.1365-2958.1994.tb01309.x
Abstract
MRNA degradation in Escherichia coli is mediated by a combination of exo- and endoribonucleases. We present evidence for a multiprotein complex which includes at least two enzymes that play important roles in mRNA degradation: the exoribonuclease polynucleotide phosphorylase (PNPase) and the endoribonuclease RNase E. An activity which impedes the processive activity of PNPase at stem-loop structures also appears to be associated with the complex. This complex is estimated to have a molecular mass of about 500 kDa and includes several additional polypeptides whose functions are unknown. The identification of a complex which includes several activities associated with mRNA degradation has implications for the mechanisms and co-ordinated control of mRNA degradation.Keywords
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