The Platelet-Fibrin Relationship in Human Blood Clots: An Ultrastructural Study Utilizing Ferritin-Conjugated Anti-Human Fibrinogen Antibody

Abstract

Platelets from re-calcified human plasma, exposed to 2C, treated wife osmic acid, dried, and sectioned, show a number of dense Alpha granules which form an asurophilic mass in fee center of platelets undergoing viscous metamorphosis. Fibrin strands appeared to be connected to fee altered alpha granulomere of these platelets. Clots formed from platelet-rich plasma preincubated wife ferritin-conjugated anti-human fibrinogen antibody contained altered platelets wife an intracellular distribution of fee ferritin conjugated antibody. Connections between these intracellular structures and fibrin strands banded by fee f erritin stain, were noted.