Genetic analysis and molecular cloning of the Escherichia coli ruv gene
- 1 April 1984
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 194 (1-2), 322-329
- https://doi.org/10.1007/bf00383535
Abstract
The genetic organisation of the ruv gene, a component of the SOS system for DNA repair and recombination in Escherichia coli, was investigated. New point mutations as well as insertions and deletions were generated using transposon Tn10 inserted in eda as a linked marker for site specific mutagenesis, or directly as a mutagen. The mutations were ordered with respect to one another and previously isolated ruv alleles by means of transductional crosses. The direction of chromosome mobilization from ruv:: Mud(ApR lac)strains carrying F42lac + established that ruv is transcribed in a counterclockwise direction. Recombinant λ phages able to restore UV resistance to ruv mutants were identified, and the ruv + region was subcloned into a low copy number plasmid. The ruv + plasmid was able to correct the extreme radiation sensitivity and recombination deficiency of ruv recBC sbcB strains.This publication has 44 references indexed in Scilit:
- Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN productMolecular Genetics and Genomics, 1984
- The E. coli uvrD gene product is DNA helicase IIMolecular Genetics and Genomics, 1983
- A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged regionCell, 1983
- Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K12Molecular Genetics and Genomics, 1983
- The Escherichia coli uvrD gene is inducible by DNA damageMolecular Genetics and Genomics, 1983
- Cloning of the uvrD gene of E. coli and identification of the productGene, 1982
- The SOS regulatory system of Escherichia coliCell, 1982
- DNA Repair in Bacteria and Mammalian CellsAnnual Review of Biochemistry, 1979
- Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10Journal of Molecular Biology, 1979
- Detection of transcribable recombination products following conjugation in Rec+, RecB− and RecC− strains of Escherichia coli K12Journal of Molecular Biology, 1974