NUCLEAR MAGNETIC RESONANCE STUDIES OF THE STRUCTURE AND BINDING SITES OF ENZYMES, X. PREPARATION OF SELECTIVELY DEUTERATED ANALOGS OF STAPHYLOCOCCAL NUCLEASE

Abstract

Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The (1)H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonated sites.