Elimination of bacteria on different implant surfaces through photosensitization and soft laser. An in vitro study.

Abstract

Microbiologic examinations of implants have shown that certain microorganisms described as periodontal pathogens may have an influence on the development and the progression of peri-implant disease. This experimental study aimed to examine the bactericidal effect of irradiation with a soft laser on bacteria associated with peri-implantitis following exposure to a photosensitizing substance. Platelets made of commercially pure titanium, either with a machined surface or with a hydroxyapatite or plasma-flame-sprayed surface or with a corundum-blasted and etched surface, were incubated with a pure suspension of Actinobacillus actinomycetemcomitans or Porphyromonas gingivalis or Prevotella intermedia. The surfaces were then treated with a toluidine blue solution and irradiated with a diode soft laser with a wave length of 905 nm for 1 min. None of the smears obtained from the thus treated surfaces showed bacterial growth, whereas the smears obtained from surfaces that had been subjected to only one type of treatment showed unchanged growth of every target organism tested (P < 0.0006). Electron microscopic inspection of the thus treated platelets revealed that combined dye/laser treatment resulted in the destruction of bacterial cells. The present in vitro results indicate that lethal photosensitization may be of use for treatment of peri-implantitis.