Production of Human Lymphoblastoid Interferon by Namalwa Cells Cultured in Serum-free Media

Abstract
The human lymphoblastoid cell line, Namalwa, can be cultured in serum-free media to cell densities of 3 to 4 × 106 cells per ml. These cultures produce up to 10000 units of interferon per ml when induced with Newcastle disease virus, strain B1. Maximum accumulation of interferon was obtained at approx. 13 h post-induction.