Calcium‐Dependent, Tetrodotoxin‐Sensitive Stimulation of Cortical Serotonin Release After a Tryptophan Load
- 1 September 1989
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 53 (3), 976-978
- https://doi.org/10.1111/j.1471-4159.1989.tb11802.x
Abstract
The effect of intraperitoneal administration of tryptophan (50, 100, or 200 mg/kg) on extracellular concentrations of tryptophan, serotonin (5-hydroxytryptamine, 5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) was studied in the cortex of freely moving rats by transcerebral dialysis. Rats were implanted with dialysis probes in the frontal cortex, and experiments were performed 24 h later. Tryptophan, 5-HT, and 5-HIAA were quantified in 20-min samples of dialysate by HPLC with electrochemical detection after separation on reverse-phase columns. Tryptophan administration resulted in a significant increase of tryptophan, 5-HT, and 5-HIAA levels in di-alysates. The maximal increase of 5-HT and 5-HIAA output was ˜ 150% over basal values. Perfusion with Ringer's solution containing tetrodotoxin (1 μM) reduced 5-HT output by 90% and prevented the increase of 5-HT and 5-HIAA content after 100 mg/kg of tryptophan. Similar results were obtained after perfusion with Ringer's solution without Ca2+. The results indicate that a tryptophan load stimulates the physiological release of 5-HT.Keywords
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