Immunohistochemical Study of Normal Skin of HIV-1—Infected Patients Shows No Evidence of Infection of Epidermal Langerhans Cells by HIV
- 1 June 1989
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 5 (3), 293-302
- https://doi.org/10.1089/aid.1989.5.293
Abstract
Langerhans cells (LC) are dendritic epidermal antigen-presenting cells expressing the surface molecule CD4, which renders them theoretical cellular targets for direct infection by the human immunodeficiency virus (HIV). To date, somewhat conflicting results have been reported concerning the in vivo infection of LC by HIV as well as the numerical alteration of these cells in the course of HIV infection. In the present work we studied clinically normal skin of a group of 44 HIV-1—seropositive patients classified according to the Centers for Disease Control (CDC) stages II (n = 14), III (n = 9), and IV (n = 21). Monoclonal antibodies (MAb) to HIV p18, p24, and gp120 and to HLA-DR and CD1a antigens (specific for LC) were applied on frozen skin sections using an amplification biotin-streptavidin-fluorescein technique. The MAb to HIV p18 cross-reacted with a cytoplasmic antigen of epidermal basal keratinocytes also present on HIV-seronegative skin specimens. No other reactivity was observed with any of the three anti-HIV MAb. The quantitative study showed that no significant correlations could be established between the number of LC (evaluated independently by HLA-DR and CD1a antigens) and the number of peripheral blood CD4+ve lymphocytes or the CDC disease stage. These results cast some doubt on the previously reported in vivo infection and numerical decrease in LC in HIV infection. The precise involvement of LC in HIV infection awaits further investigation.This publication has 30 references indexed in Scilit:
- Pathogenesis of Infection with Human Immunodeficiency VirusNew England Journal of Medicine, 1987
- Predictors of the Acquired Immunodeficiency Syndrome Developing in a Cohort of Seropositive Homosexual MenNew England Journal of Medicine, 1987
- The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brainCell, 1986
- T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAVNature, 1984
- The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirusNature, 1984
- An assessment of Langerhans cell quantification in tissue sectionsJournal of the American Academy of Dermatology, 1984
- Quantitative evaluation of two distinct cell populations expressing HLA-DR antigens in normal human epidermisBritish Journal of Dermatology, 1984
- Reduced Langerhans’ Cell Ia Antigen and ATPase Activity in Patients with the Acquired Immunodeficiency SyndromeNew England Journal of Medicine, 1984
- Quantitative variation in la antigen expression plays a central role in immune regulationImmunology Today, 1984
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983