Molecular Phylogeny of Anabaena circinalis and Its Identification in Environmental Samples by PCR
- 1 September 2000
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 66 (9), 4145-4148
- https://doi.org/10.1128/aem.66.9.4145-4148.2000
Abstract
Although the cyanobacterium Anabaena circinalis occurs worldwide, Australian isolates are believed to exclusively possess the saxitoxin group neurotoxins (paralytic shellfish poisons). Identification of A. circinalis in a mixed population is complicated due to limited morphological differences between Anabaena species. Sequence analysis of the DNA-dependent RNA polymerase ( rpoC1 ) gene from 24 Anabaena isolates, including 12 designated A. circinalis , permitted a phylogenetic analysis to be performed. In addition, an A. circinalis -specific PCR was developed and tested successfully on environmental samples.Keywords
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