Localization of the immunoglobulins G, A and M, β -trace protein and γ -trace protein on isoelectric focusing of serum and cerebrospinal fluid by immunofixation

Abstract

The mobility of the immunoglobulins G, A and M, β-trace protein and γ-trace protein on isoelectric focusing of serum and CSF was determined by immunofixation using specific antisera. Polyclonal IgG migrated as multiple bands between pH 4.7–8.6, polyclonal IgA as multiple bands between pH 4.9–6.1 in CSF and serum. IgM could not be identified in normal CSF or serum. β-trace protein gave three bands at pH 8.0, 8.4 and 7.4–7.5, respectively, while γ-trace protein gave one single band at pH 9.5-> 9.5. Oligoclonal IgG in CSF in multiple sclerosis and neurosyphilis migrated between pH 8.6-> 9.5 and was easily discriminated from other proteins.